Papers describing expression of various proteins (chronological list):
- Kang L, Chen X, Zhai C, Ma L. Synthesis and High Expression of Chitin Deacetylase from Colletotrichum lindemuthianum in Pichia pastoris GS115. J Microbiol Biotechnol. 2012 Sep;22(9):1202-7. PMID: 22814492
- Xia WR, Fu WL, Cai L, Cai X, Wang YY, Zou MJ, Xu DG. Expression, Purification and Characterization of Recombinant Human Angiogenin in Pichia pastoris. Biosci Biotechnol Biochem. 2012 Jul 23;76(7):1384-8. Epub 2012 Jul 7. PMID: 22785483
- Fan G, Katrolia P, Jia H, Yang S, Yan Q, Jiang Z. High-level expression of a xylanase gene from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris. Biotechnol Lett. 2012 Jul 11. [Epub ahead of print] PMID: 22782269
- Zhu RY, Xin X, Dai HY, Li Q, Lei JY, Chen Y, Jin J. Expression and purification of recombinant human serum albumin fusion protein with VEGF165b in Pichia pastoris. Protein Expr Purif. 2012 Jun 28;85(1):32-37. [Epub ahead of print] PMID: 22750397
- Schoonooghe S. Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris. Methods Mol Biol. 2012;899:157-75. PubMed PMID: 22735952
- Koliasnikov OV, Grigorenko VG, Egorov AM, Lange S, Schmid RD. Recombinant Production of Horseradish Peroxidase Conjugates with Fab Antibodies in Pichia pastoris for Analytical Applications. Acta Naturae. 2011 Jul;3(3):85-92. PMID: 22649698
- Lei J, Guan B, Li B, Duan Z, Chen Y, Li H, Jin J. Expression, purification and characterization of recombinant human interleukin-2-serum albumin (rhIL-2-HSA) fusion protein in Pichia pastoris. Protein Expr Purif. 2012 Jul;84(1):154-60. Epub 2012 May 17. PMID: 22609631
- Mellitzer A, Weis R, Glieder A, Flicker K. Expression of lignocellulolytic enzymes in Pichia pastoris. Microb Cell Fact. 2012 May 14;11(1):61. [Epub ahead of print] PMID: 22583625
- Choi BK, Warburton S, Lin H, Patel R, Boldogh I, Meehl M, d’Anjou M, Pon L, Stadheim TA, Sethuraman N. Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris. Appl Microbiol Biotechnol. 2012 Aug;95(3):671-82. Epub 2012 May 10. PMID: 22569635
- Kukk K, Järving R, Samel N. Purification and characterization of the recombinant human prostaglandin H synthase-2 expressed in Pichia pastoris. Protein Expr Purif. 2012 Jun;83(2):182-9. Epub 2012 Apr 5 PMID: 22504626
- Rothan HA, Teh SH, Haron K, Mohamed Z. A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli. Int J Mol Sci. 2012;13(3):3549-62. Epub 2012 Mar 15. PMID: 22489167
- Miller J, Doss M, McQuillen R, Shaller CC, Tolner B, Yu JQ, Chester K, Robinson MK. Impact of expression system on the function of the C6.5 diabody PET radiotracer. Tumour Biol. 2012 Jun;33(3):617-27. Epub 2012 Mar 1. PubMed PMID: 22383295
- Pfeffer M, Maurer M, Stadlmann J, Grass J, Delic M, Altmann F, Mattanovich D. Intracellular interactome of secreted antibody Fab fragment in Pichia pastoris reveals its routes of secretion and degradation. Appl Microbiol Biotechnol. 2012 Mar;93(6):2503-12. Epub 2012 Feb 18. PubMed PMID: 22350260.
- Ezzine A, M’hirsi El Adab S, Bouhaouala-Zahar B, Hmila I, Baciou L, Marzouki,MN. Efficient expression of the anti-AahI’ scorpion toxin nanobody under a new functional form in a Pichia pastoris system. Biotechnol Appl Biochem. 2012 Jan-Feb;59(1):15-21. doi: 10.1002/bab.67. Epub 2012 Jan 18. PubMed PMID: 22332740
- Krainer FW, Dietzsch C, Hajek T, Herwig C, Spadiut O, Glieder A. Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway. Microb Cell Fact. 2012 Feb 13;11:22. PubMed PMID: 22330134; PubMed Central PMCID: PMC3295664.
- Peraino J, Zhang H, Hermanrud CE, Li G, Sachs DH, Huang CA, Wang Z. Expression and purification of soluble porcine CTLA-4 in yeast Pichia pastoris. Protein Expr Purif. 2012 Apr;82(2):270-8. Epub 2012 Feb 2. PMID: 22326797
- Bordas-Le Floch V, Bussières L, Airouche S, Lautrette A, Bouley J, Berjont N, Horiot S, Huet A, Jain K, Lemoine P, Chabre H, Batard T, Mascarell L, Baron-Bodo V, Tourdot S, Nony E, Moingeon P. Expression and Characterization of Natural-Like Recombinant Der p 2 for Sublingual Immunotherapy. Int Arch Allergy Immunol. 2012;158(2):157-67. Epub 2012 Jan 25. PubMed PMID: 22286395.
- Mattanovich D, Branduardi P, Dato L, Gasser B, Sauer M, Porro D. Recombinant protein production in yeasts. Methods Mol Biol. 2012;824:329-58. Review. PubMed PMID: 22160907.
- Nett JH, Gomathinayagam S, Hamilton SR, Gong B, Davidson RC, Du M, Hopkins D, Mitchell T, Mallem MR, Nylen A, Shaikh SS, Sharkey N, Barnard GC, Copeland V, Liu L, Evers R, Li Y, Gray PM, Lingham RB, Visco D, Forrest G, DeMartino J, Linden T, Potgieter TI, Wildt S, Stadheim TA, d’Anjou M, Li H, Sethuraman N. Optimization of erythropoietin production with controlled glycosylation-PEGylated erythropoietin produced in glycoengineered Pichia pastoris. J Biotechnol. 2012 Jan;157(1):198-206. Epub 2011 Nov 9. PubMed PMID: 22100268.
- Ha S, Wang Y, Rustandi RR. Biochemical and biophysical characterization of humanized IgG1 produced in Pichia pastoris. MAbs. 2011 Sep-Oct;3(5):453-60. Epub 2011 Sep 1. Review. PubMed PMID: 22048694; PubMed Central PMCID: PMC3225849.
- Austin RJ, Kuestner RE, Chang DK, Madden KR, Martin DB. SILAC compatible strain of Pichia pastoris for expression of isotopically labeled protein standards and quantitative proteomics. J Proteome Res. 2011 Nov 4;10(11):5251-9. Epub 2011 Oct 14. PubMed PMID: 21942632; PubMed Central PMCID: PMC3361967.
- Teh SH, Fong MY, Mohamed Z. Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris. Genet Mol Biol. 2011 Jul;34(3):464-70. Epub 2011 Jul 1. PubMed PMID: 21931521; PubMed Central PMCID: PMC3168189.
- Li H, Li N, Gao X, Kong X, Li S, Xu A, Jin S, Wu D. High level expression of active recombinant human interleukin-3 in Pichia pastoris. Protein Expr Purif. 2011 Dec;80(2):185-93. Epub 2011 Aug 31. PubMed PMID: 21907288.
- Lünsdorf H, Gurramkonda C, Adnan A, Khanna N, Rinas U. Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen. Microb Cell Fact. 2011 Jun 26;10:48. PubMed PMID: 21703024; PubMed Central PMCID:PMC3142206.
- Bruce C, Fitches EC, Chougule N, Bell HA, Gatehouse JA. Recombinant conotoxin, TxVIA, produced in yeast has insecticidal activity. Toxicon. 2011 Jul;58(1):93-100. Epub 2011 May 27. PubMed PMID: 21640131.
- Sommaruga S, Lombardi A, Salvadè A, Mazzucchelli S, Corsi F, Galeffi P, Tortora P, Prosperi D. Highly efficient production of anti-HER2 scFv antibody variant for targeting breast cancer cells. Appl Microbiol Biotechnol. 2011 Aug;91(3):613-21. Epub 2011 May 3. PubMed PMID: 21538107.
- Li H, Wang Y, Xu A, Li S, Jin S, Wu D. Large-scale production, purification and bioactivity assay of recombinant human interleukin-6 in the methylotrophic yeast Pichia pastoris. FEMS Yeast Res. 2011 Mar;11(2):160-7. doi: 10.1111/j.1567-1364.2010.00701.x. Epub 2010 Dec 22. PubMed PMID: 21176103.
- Barnard GC, Kull AR, Sharkey NS, Shaikh SS, Rittenhour AM, Burnina I, Jiang Y, Li F, Lynaugh H, Mitchell T, Nett JH, Nylen A, Potgieter TI, Prinz B, Rios SE, Zha D, Sethuraman N, Stadheim TA, Bobrowicz P. High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies. J Ind Microbiol Biotechnol. 2010 Sep;37(9):961-71. Epub 2010 Aug 15. PubMed PMID: 20711797.
- Potgieter TI, Kersey SD, Mallem MR, Nylen AC, d’Anjou M. Antibody expression kinetics in glycoengineered Pichia pastoris. Biotechnol Bioeng. 2010 Aug 15;106(6):918-27. PubMed PMID: 20506148.
- Majidzadeh-A K, Khalaj V, Fatemeh D, Mahdi H, Farzaneh B, Ahmad A, Mahboudi F. Cloning and expression of functional full-length human tissue plasminogen activator in Pichia pastoris. Appl Biochem Biotechnol. 2010 Nov;162(7):2037-48. Epub 2010 May 9. PubMed PMID: 20455033.
- Lee CH, Choi DK, Choi HJ, Song MY, Kim YS. Expression of soluble and functional human neonatal Fc receptor in Pichia pastoris. Protein Expr Purif. 2010 May;71(1):42-8. Epub 2009 Dec 16. PubMed PMID: 20006709.
- Ryckaert S, Pardon E, Steyaert J, Callewaert N. Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris. J Biotechnol. 2010 Jan 15;145(2):93-8. Epub 2009 Oct 25. PubMed PMID: 19861136.
- Apte-Deshpande A, Rewanwar S, Kotwal P, Raiker VA, Padmanabhan S. Efficient expression and secretion of recombinant human growth hormone in the expression and secretion of recombinant human growth hormone in the methylotrophic yeast Pichia pastoris: potential applications for other proteins. Biotechnol Appl Biochem. 2009 Nov 13;54(4):197-205. PubMed PMID: 19814714.
- Pokoj S, Lauer I, Fötisch K, Himly M, Mari A, Enrique E, Miguel-Moncin Mdel M, Lidholm J, Vieths S, Scheurer S. Pichia pastoris is superior to E. coli for the production of recombinant allergenic non-specific lipid-transfer proteins. Protein Expr Purif. 2010 Jan;69(1):68-75. Epub 2009 Sep 3. PubMed PMID: 19733242.
- Bhanot V, Balamurugan V, Bhanuprakash V, Venkatesan G, Sen A, Yadav V, Yogisharadhya R, Singh RK. Expression of P32 protein of goatpox virus in Pichia pastoris and its potential use as a diagnostic antigen in ELISA. J Virol Methods. 2009 Dec;162(1-2):251-7. Epub 2009 Sep 3. PubMed PMID: 19733197.
- Potgieter TI, Cukan M, Drummond JE, Houston-Cummings NR, Jiang Y, Li F, Lynaugh H, Mallem M, McKelvey TW, Mitchell T, Nylen A, Rittenhour A, Stadheim TA, Zha D, d’Anjou M. Production of monoclonal antibodies by glycoengineered Pichia pastoris. J Biotechnol. 2009 Feb 23;139(4):318-25. Epub 2008 Dec 27. PubMed PMID: 19162096
- Kong N, Mu X, Han H, Yan W. Pilot-scale fermentation, purification, and characterization of recombinant human Oncostatin M in Pichia pastoris. Protein Expr Purif. 2009 Feb;63(2):134-9. Epub 2008 Oct 14. PubMed PMID: 18957323.
- Cai H, Chen L, Wan L, Zeng L, Yang H, Li S, Li Y, Cheng J, Lu X. High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris. Appl Microbiol Biotechnol. 2009 Feb;82(1):41-8. Epub 2008 Oct 21. PubMed PMID: 18936937.
- Xie T, Liu Q, Xie F, Liu H, Zhang Y. Secretory expression of insulin precursor in pichia pastoris and simple procedure for producing recombinant human insulin. Prep Biochem Biotechnol. 2008;38(3):308-17. PubMed PMID: 18569877.
- Khasa YP, Khushoo A, Srivastava L, Mukherjee KJ. Kinetic studies of constitutive human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression in continuous culture of Pichia pastoris. Biotechnol Lett. 2007 Dec;29(12):1903-8. Epub 2007 Jul 25. PubMed PMID: 17653623.
- Hao Y, Chu J, Wang Y, Zhang S, Zhuang Y. Expression and aggregation of recombinant human consensus interferon-alpha mutant by Pichia pastoris. Biotechnol Lett. 2006 Jun;28(12):905-9. Epub 2006 May 31. PubMed PMID: 16786276.
- Feng MQ, Cai QS, Song DX, Dong JB, Zhou P. High yield and secretion of recombinant human apolipoprotein AI in Pichia pastoris. Protein Expr Purif. 2006 Apr;46(2):337-42. Epub 2005 Dec 12. PubMed PMID: 16516487.
- Hu S, Li L, Qiao J, Guo Y, Cheng L, Liu J. Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris. Protein Expr Purif. 2006 May;47(1):249-57. Epub 2005 Dec 13. PubMed PMID: 16403645.
- Qu J, Li X, Huang B. Production of recombinant human microplasminogen and pilot study in inducing posterior vitreous detachment. Curr Eye Res. 2005 Oct;30(10):881-9. PubMed PMID: 16251125.
- Kobayashi K, Kuwae S, Ohya T, Ohda T, Ohyama M, Ohi H, Tomomitsu K, Ohmura T. High-level expression of recombinant human serum albumin from the activation. J Biosci Bioeng. 2000;89(1):55-61. PubMed PMID: 16232698.
- Lenassi Zupan A, Trobec S, Gaberc-Porekar V, Menart V. High expression of green fluorescent protein in Pichia pastoris leads to formation of fluorescent particles. J Biotechnol. 2004 Apr 8;109(1-2):115-22. PubMed PMID: 15063619.
- Cregg JM, Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T. Expression in the yeast Pichia pastoris. Methods Enzymol. 2009;463:169-89. Review. PubMed PMID: 19892173.