Welcome to Pichia.com - Simple, Secreted, Scalable, Eukaryotic Protein Expression Platform x


Pichia is a widely used and well published system for cost-effective protein production We have selected papers on Pichia expresssion from a collection of over 2500 papers and segmented them by category as follows:

  • Types of Proteins: Publications demonstrating utility of Pichia pastoris for the production of a number of different protein types
  • Techniques: Publications on expression techniques using Pichia.
  • Fermentation: Publications on Pichia expression during fermentation

A comprehensive description of RCT’s Pichia Expression Platform that includes fermentation strategies for Pichia is featured in Christian Julien’s January 2006 BioProcess article.

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Chronological List of Selected Papers on Pichia Expresssion (from a list of over 2500 papers)
1Vogl T, Hartner FS, Glieder A. New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris. Curr Opin Biotechnol. 2013 Mar 19 [Epub ahead of print] PMID: 23522654
2Kang L, Chen X, Zhai C, Ma L. Synthesis and High Expression of Chitin Deacetylase from Colletotrichum lindemuthianum in Pichia pastoris GS115. J Microbiol Biotechnol. 2012 Sep;22(9):1202-7. PMID: 22814492
3Xia WR, Fu WL, Cai L, Cai X, Wang YY, Zou MJ, Xu DG. Expression, Purification and Characterization of Recombinant Human Angiogenin in Pichia pastoris. Biosci Biotechnol Biochem. 2012 Jul 23;76(7):1384-8. Epub 2012 Jul 7. PMID: 22785483
4Fan G, Katrolia P, Jia H, Yang S, Yan Q, Jiang Z. High-level expression of a xylanase gene from the thermophilic fungus Paecilomyces thermophila in Pichia pastoris. Biotechnol Lett. 2012 Jul 11. [Epub ahead of print] PMID: 22782269
5Zhu RY, Xin X, Dai HY, Li Q, Lei JY, Chen Y, Jin J. Expression and purification of recombinant human serum albumin fusion protein with VEGF165b in Pichia pastoris. Protein Expr Purif. 2012 Jun 28;85(1):32-37. [Epub ahead of print] PMID: 22750397
6Chen MT, Lin S, Shandil I, Andrews D, Stadheim TA, Choi BK. Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production. Microb Cell Fact. 2012 Jul 2;11(1):91. [Epub ahead of print] PMID: 22748191
7Schoonooghe S. Engineering and Expression of Bibody and Tribody Constructs in Mammalian Cells and in the Yeast Pichia pastoris. Methods Mol Biol. 2012;899:157-75. PubMed PMID: 22735952
8Carnicer M, Pierich AT, Dam JV, Heijnen JJ, Albiol J, Gulik WV, Ferrer P. Quantitative metabolomics analysis of amino acid metabolism in recombinant pichia pastoris under different oxygen availability conditions. Microb Cell Fact. 2012 Jun 15;11(1):83. [Epub ahead of print] PubMed PMID: 22704468.
9Koliasnikov OV, Grigorenko VG, Egorov AM, Lange S, Schmid RD. Recombinant Production of Horseradish Peroxidase Conjugates with Fab Antibodies in Pichia pastoris for Analytical Applications. Acta Naturae. 2011 Jul;3(3):85-92. PMID: 22649698
10Lei J, Guan B, Li B, Duan Z, Chen Y, Li H, Jin J. Expression, purification and characterization of recombinant human interleukin-2-serum albumin (rhIL-2-HSA) fusion protein in Pichia pastoris. Protein Expr Purif. 2012 Jul;84(1):154-60. Epub 2012 May 17. PMID: 22609631
11Mellitzer A, Weis R, Glieder A, Flicker K. Expression of lignocellulolytic enzymes in Pichia pastoris. Microb Cell Fact. 2012 May 14;11(1):61. [Epub ahead of print] PMID: 22583625
12Choi BK, Warburton S, Lin H, Patel R, Boldogh I, Meehl M, d'Anjou M, Pon L, Stadheim TA, Sethuraman N. Improvement of N-glycan site occupancy of therapeutic glycoproteins produced in Pichia pastoris. Appl Microbiol Biotechnol. 2012 Aug;95(3):671-82. Epub 2012 May 10. PMID: 22569635
13Yang J, Yan X, Huang R, Zhang B. Codon optimization, expression and enzymatic comparison of Rhizopus oryzae lipases pro-ROL and m-ROL in Pichia pastoris. Sheng Wu Gong Cheng Xue Bao. 2011 Dec;27(12):1780-8. PMID: 22506419
14Zalai D, Dietzsch C, Herwig C, Spadiut O. A dynamic fed batch strategy for a Pichia pastoris mixed feed system to increase process understanding. Biotechnol Prog. 2012 May;28(3):878-86. doi: 10.1002/btpr.1551. Epub 2012 May 21. PMID: 22505140
15Kukk K, Järving R, Samel N. Purification and characterization of the recombinant human prostaglandin H synthase-2 expressed in Pichia pastoris. Protein Expr Purif. 2012 Jun;83(2):182-9. Epub 2012 Apr 5 PMID: 22504626
16Rothan HA, Teh SH, Haron K, Mohamed Z. A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli. Int J Mol Sci. 2012;13(3):3549-62. Epub 2012 Mar 15. PMID: 22489167
17Singh S, Gras A, Fiez-Vandal C, Martinez M, Wagner R, Byrne B. Large-scale production of membrane proteins in Pichia pastoris: the production of G protein-coupled receptors as a case study. Methods Mol Biol. 2012;866:197-207. PMID: 22454125
18Logez C, Alkhalfioui F, Byrne B, Wagner R. Preparation of Pichia pastoris expression plasmids. Methods Mol Biol. 2012;866:25-40. PMID: 22454111
19Miller J, Doss M, McQuillen R, Shaller CC, Tolner B, Yu JQ, Chester K, Robinson MK. Impact of expression system on the function of the C6.5 diabody PET radiotracer. Tumour Biol. 2012 Jun;33(3):617-27. Epub 2012 Mar 1. PubMed PMID: 22383295
20Pfeffer M, Maurer M, Stadlmann J, Grass J, Delic M, Altmann F, Mattanovich D. Intracellular interactome of secreted antibody Fab fragment in Pichia pastoris reveals its routes of secretion and degradation. Appl Microbiol Biotechnol. 2012 Mar;93(6):2503-12. Epub 2012 Feb 18. PubMed PMID: 22350260.
21Ezzine A, M'hirsi El Adab S, Bouhaouala-Zahar B, Hmila I, Baciou L, Marzouki,MN. Efficient expression of the anti-AahI' scorpion toxin nanobody under a new functional form in a Pichia pastoris system. Biotechnol Appl Biochem. 2012 Jan-Feb;59(1):15-21. doi: 10.1002/bab.67. Epub 2012 Jan 18. PubMed PMID: 22332740
22Krainer FW, Dietzsch C, Hajek T, Herwig C, Spadiut O, Glieder A. Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway. Microb Cell Fact. 2012 Feb 13;11:22. PubMed PMID: 22330134; PubMed Central PMCID: PMC3295664.
23Peraino J, Zhang H, Hermanrud CE, Li G, Sachs DH, Huang CA, Wang Z. Expression and purification of soluble porcine CTLA-4 in yeast Pichia pastoris. Protein Expr Purif. 2012 Apr;82(2):270-8. Epub 2012 Feb 2. PMID: 22326797
24Schaefer JV, Plückthun A. Engineering aggregation resistance in IgG by two independent mechanisms: lessons from comparison of Pichia pastoris and mammalian cell expression. J Mol Biol. 2012 Apr 6;417(4):309-35. Epub 2012 Jan 27. PubMed PMID: 22306407.
25Bordas-Le Floch V, Bussières L, Airouche S, Lautrette A, Bouley J, Berjont N, Horiot S, Huet A, Jain K, Lemoine P, Chabre H, Batard T, Mascarell L, Baron-Bodo V, Tourdot S, Nony E, Moingeon P. Expression and Characterization of Natural-Like Recombinant Der p 2 for Sublingual Immunotherapy. Int Arch Allergy Immunol. 2012;158(2):157-67. Epub 2012 Jan 25. PubMed PMID: 22286395.
26Mattanovich D, Branduardi P, Dato L, Gasser B, Sauer M, Porro D. Recombinant protein production in yeasts. Methods Mol Biol. 2012;824:329-58. Review. PubMed PMID: 22160907.
27Du M, Battles MB, Nett JH. A color-based stable multi-copy integrant selection system for Pichia pastoris using the attenuated ADE1 and ADE2 genes as auxotrophic markers. Bioeng Bugs. 2012 Jan 1;3(1):32-7. doi: 10.4161/bbug.3.1.17936. Epub 2012 Jan 1. PubMed PMID: 22126802.
28Nett JH, Gomathinayagam S, Hamilton SR, Gong B, Davidson RC, Du M, Hopkins D, Mitchell T, Mallem MR, Nylen A, Shaikh SS, Sharkey N, Barnard GC, Copeland V, Liu L, Evers R, Li Y, Gray PM, Lingham RB, Visco D, Forrest G, DeMartino J, Linden T, Potgieter TI, Wildt S, Stadheim TA, d'Anjou M, Li H, Sethuraman N. Optimization of erythropoietin production with controlled glycosylation-PEGylated erythropoietin produced in glycoengineered Pichia pastoris. J Biotechnol. 2012 Jan;157(1):198-206. Epub 2011 Nov 9. PubMed PMID: 22100268.
29Baumann K, Adelantado N, Lang C, Mattanovich D, Ferrer P. Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris. Microb Cell Fact. 2011 Nov 3;10:93. PubMed PMID: 22050768; PubMed Central PMCID: PMC3219557.
30Ha S, Wang Y, Rustandi RR. Biochemical and biophysical characterization of humanized IgG1 produced in Pichia pastoris. MAbs. 2011 Sep-Oct;3(5):453-60. Epub 2011 Sep 1. Review. PubMed PMID: 22048694; PubMed Central PMCID: PMC3225849.
31Lin S, Houston-Cummings NR, Prinz B, Moore R, Bobrowicz B, Davidson RC, Wildt S, Stadheim TA, Zha D. A novel fragment of antigen binding (Fab) surface display platform using glycoengineered Pichia pastoris. J Immunol Methods. 2012 Jan 31;375(1-2):159-65. Epub 2011 Oct 12. PubMed PMID: 22019510.
32Austin RJ, Kuestner RE, Chang DK, Madden KR, Martin DB. SILAC compatible strain of Pichia pastoris for expression of isotopically labeled protein standards and quantitative proteomics. J Proteome Res. 2011 Nov 4;10(11):5251-9. Epub 2011 Oct 14. PubMed PMID: 21942632; PubMed Central PMCID: PMC3361967.
33Teh SH, Fong MY, Mohamed Z. Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris. Genet Mol Biol. 2011 Jul;34(3):464-70. Epub 2011 Jul 1. PubMed PMID: 21931521; PubMed Central PMCID: PMC3168189.
34Wu D, Chu J, Hao YY, Wang YH, Zhuang YP, Zhang SL. Incomplete protein disulphide bond conformation and decreased protein expression result from high cell growth during heterologous protein expression in Pichia pastoris. J Biotechnol. 2012 Jan;157(1):107-12. Epub 2011 Sep 8. PubMed PMID: 21924302.
35Li H, Li N, Gao X, Kong X, Li S, Xu A, Jin S, Wu D. High level expression of active recombinant human interleukin-3 in Pichia pastoris. Protein Expr Purif. 2011 Dec;80(2):185-93. Epub 2011 Aug 31. PubMed PMID: 21907288.
36Akcapinar GB, Gul O, Sezerman U. Effect of codon optimization on the expression of Trichoderma reesei endoglucanase 1 in Pichia pastoris. Biotechnol Prog. 2011 Sep-Oct;27(5):1257-63. doi: 10.1002/btpr.663. Epub 2011 Jul 19. PubMed PMID: 21774095.
37Lünsdorf H, Gurramkonda C, Adnan A, Khanna N, Rinas U. Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producing high levels of the hepatitis B surface antigen. Microb Cell Fact. 2011 Jun 26;10:48. PubMed PMID: 21703024; PubMed Central PMCID:PMC3142206.
38Bruce C, Fitches EC, Chougule N, Bell HA, Gatehouse JA. Recombinant conotoxin, TxVIA, produced in yeast has insecticidal activity. Toxicon. 2011 Jul;58(1):93-100. Epub 2011 May 27. PubMed PMID: 21640131.
39Sommaruga S, Lombardi A, Salvadè A, Mazzucchelli S, Corsi F, Galeffi P, Tortora P, Prosperi D. Highly efficient production of anti-HER2 scFv antibody variant for targeting breast cancer cells. Appl Microbiol Biotechnol. 2011 Aug;91(3):613-21. Epub 2011 May 3. PubMed PMID: 21538107.
40Fricke J, Pohlmann K, Tatge F, Lang R, Faber B, Luttmann R. A multi-bioreactor system for optimal production of malaria vaccines with Pichia pastoris. Biotechnol J. 2011 Apr;6(4):437-51. doi: 10.1002/biot.201000385. PubMed PMID: 21472987.
41Li H, Wang Y, Xu A, Li S, Jin S, Wu D. Large-scale production, purification and bioactivity assay of recombinant human interleukin-6 in the methylotrophic yeast Pichia pastoris. FEMS Yeast Res. 2011 Mar;11(2):160-7. doi: 10.1111/j.1567-1364.2010.00701.x. Epub 2010 Dec 22. PubMed PMID: 21176103.
42Jacobs PP, Inan M, Festjens N, Haustraete J, Van Hecke A, Contreras R, Meagher MM, Callewaert N. Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate. Microb Cell Fact. 2010 Nov 23;9:93. PubMed PMID: 21092289; PubMed Central PMCID: PMC3004841.
43Baumann K, Carnicer M, Dragosits M, Graf AB, Stadlmann J, Jouhten P, Maaheimo H, Gasser B, Albiol J, Mattanovich D, Ferrer P. A multi-level study of recombinant Pichia pastoris in different oxygen conditions. BMC Syst Biol. 2010 Oct 22;4:141. PubMed PMID: 20969759; PubMed Central PMCID: PMC2987880.
44Stadlmayr G, Mecklenbräuker A, Rothmüller M, Maurer M, Sauer M, Mattanovich D, Gasser B. Identification and characterisation of novel Pichia pastoris promoters for heterologous protein production. J Biotechnol. 2010 Dec;150(4):519-29. Epub 2010 Oct 8. PubMed PMID: 20933554.
45Barnard GC, Kull AR, Sharkey NS, Shaikh SS, Rittenhour AM, Burnina I, Jiang Y, Li F, Lynaugh H, Mitchell T, Nett JH, Nylen A, Potgieter TI, Prinz B, Rios SE, Zha D, Sethuraman N, Stadheim TA, Bobrowicz P. High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies. J Ind Microbiol Biotechnol. 2010 Sep;37(9):961-71. Epub 2010 Aug 15. PubMed PMID: 20711797.
46Guerfal M, Ryckaert S, Jacobs PP, Ameloot P, Van Craenenbroeck K, Derycke R, Callewaert N. The HAC1 gene from Pichia pastoris: characterization and effect of proteins. Microb Cell Fact. 2010 Jun 30;9:49. PubMed PMID: 20591165; PubMed Central PMCID: PMC2905327.
47De Pourcq K, De Schutter K, Callewaert N. Engineering of glycosylation in yeast and other fungi: current state and perspectives. Appl Microbiol Biotechnol. 2010 Aug;87(5):1617-31. Epub 2010 Jun 29. Review. PMID: 20585772
48Potgieter TI, Kersey SD, Mallem MR, Nylen AC, d'Anjou M. Antibody expression kinetics in glycoengineered Pichia pastoris. Biotechnol Bioeng. 2010 Aug 15;106(6):918-27. PubMed PMID: 20506148.
49Majidzadeh-A K, Khalaj V, Fatemeh D, Mahdi H, Farzaneh B, Ahmad A, Mahboudi F. Cloning and expression of functional full-length human tissue plasminogen activator in Pichia pastoris. Appl Biochem Biotechnol. 2010 Nov;162(7):2037-48. Epub 2010 May 9. PubMed PMID: 20455033.
50Al-Samarrai TH, Kirk CA, Jones WT, Harvey D, Templeton MD. A simple and rapid method for selecting high producers of recombinant proteins in individual clones of P. pastoris. Comb Chem High Throughput Screen. 2010 Jun;13(5):377-82. PubMed PMID: 20236060.
51Vanderschaeghe D, Festjens N, Delanghe J, Callewaert N. Glycome profiling using modern glycomics technology: technical aspects and applications. Biol Chem. 2010 Feb-Mar;391(2-3):149-61. Review. PMID: 20128687
52Lee CH, Choi DK, Choi HJ, Song MY, Kim YS. Expression of soluble and functional human neonatal Fc receptor in Pichia pastoris. Protein Expr Purif. 2010 May;71(1):42-8. Epub 2009 Dec 16. PubMed PMID: 20006709.
53Cregg JM, Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T. Expression in the yeast Pichia pastoris. Methods Enzymol. 2009;463:169-89. Review. PubMed PMID: 19892173.
54Ryckaert S, Pardon E, Steyaert J, Callewaert N. Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris. J Biotechnol. 2010 Jan 15;145(2):93-8. Epub 2009 Oct 25. PubMed PMID: 19861136.
55Apte-Deshpande A, Rewanwar S, Kotwal P, Raiker VA, Padmanabhan S. Efficient expression and secretion of recombinant human growth hormone in the expression and secretion of recombinant human growth hormone in the methylotrophic yeast Pichia pastoris: potential applications for other proteins. Biotechnol Appl Biochem. 2009 Nov 13;54(4):197-205. PubMed PMID: 19814714.
56Pokoj S, Lauer I, Fötisch K, Himly M, Mari A, Enrique E, Miguel-Moncin Mdel M, Lidholm J, Vieths S, Scheurer S. Pichia pastoris is superior to E. coli for the production of recombinant allergenic non-specific lipid-transfer proteins. Protein Expr Purif. 2010 Jan;69(1):68-75. Epub 2009 Sep 3. PubMed PMID: 19733242.
57Bhanot V, Balamurugan V, Bhanuprakash V, Venkatesan G, Sen A, Yadav V, Yogisharadhya R, Singh RK. Expression of P32 protein of goatpox virus in Pichia pastoris and its potential use as a diagnostic antigen in ELISA. J Virol Methods. 2009 Dec;162(1-2):251-7. Epub 2009 Sep 3. PubMed PMID: 19733197.
58Holmes WJ, Darby RA, Wilks MD, Smith R, Bill RM. Developing a scalable model of recombinant protein yield from Pichia pastoris: the influence of culture conditions, biomass and induction regime. Microb Cell Fact. 2009 Jul 1;8:35. PubMed PMID: 19570229; PubMed Central PMCID: PMC2717918.
59Mattanovich D, Graf A, Stadlmann J, Dragosits M, Redl A, Maurer M, Kleinheinz M, Sauer M, Altmann F, Gasser B. Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. Microb Cell Fact. 2009 Jun 2;8:29. PubMed PMID: 19490607; PubMed Central PMCID: PMC2702363.
60Potgieter TI, Cukan M, Drummond JE, Houston-Cummings NR, Jiang Y, Li F, Lynaugh H, Mallem M, McKelvey TW, Mitchell T, Nylen A, Rittenhour A, Stadheim TA, Zha D, d'Anjou M. Production of monoclonal antibodies by glycoengineered Pichia pastoris. J Biotechnol. 2009 Feb 23;139(4):318-25. Epub 2008 Dec 27. PubMed PMID: 19162096
61Jacobs PP, Geysens S, Vervecken W, Contreras R, Callewaert N. Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology. Nat Protoc. 2009;4(1):58-70. PubMed PMID: 19131957.
62Kong N, Mu X, Han H, Yan W. Pilot-scale fermentation, purification, and characterization of recombinant human Oncostatin M in Pichia pastoris. Protein Expr Purif. 2009 Feb;63(2):134-9. Epub 2008 Oct 14. PubMed PMID: 18957323.
63Cai H, Chen L, Wan L, Zeng L, Yang H, Li S, Li Y, Cheng J, Lu X. High-level expression of a functional humanized anti-CTLA4 single-chain variable fragment antibody in Pichia pastoris. Appl Microbiol Biotechnol. 2009 Feb;82(1):41-8. Epub 2008 Oct 21. PubMed PMID: 18936937.
64Jacobs PP, Ryckaert S, Geysens S, De Vusser K, Callewaert N, Contreras R.Pichia surface display: display of proteins on the surface of glycoengineered Pichia pastoris strains. Biotechnol Lett. 2008 Dec;30(12):2173-81. Epub 2008 Aug 5. PubMed PMID: 18679585.
65Xie T, Liu Q, Xie F, Liu H, Zhang Y. Secretory expression of insulin precursor in pichia pastoris and simple procedure for producing recombinant human insulin. Prep Biochem Biotechnol. 2008;38(3):308-17. PubMed PMID: 18569877.
66Blanchard V, Gadkari RA, George AV, Roy S, Gerwig GJ, Leeflang BR, Dighe RR, Boelens R, Kamerling JP. High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains--selection of strain GS115, and not X-33, for the production of biologically active N-glycosylated 15N-labeled phCG. Glycoconj J. 2008 Apr;25(3):245-57. Epub 2008 Feb 15. PubMed PMID: 18274893; PubMed Central PMCID: PMC2668595.
67Lin-Cereghino J, Lin-Cereghino GP. Vectors and strains for expression. Methods Mol Biol. 2007;389:11-26. PubMed PMID: 17951632.
68Cregg JM. Introduction: distinctions between Pichia pastoris and other expression systems. Methods Mol Biol. 2007;389:1-10. Review. PubMed PMID: 17951631
69Vervecken W, Callewaert N, Kaigorodov V, Geysens S, Contreras R. Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids. Methods Mol Biol. 2007;389:119-38. PMID: 17951639
70Khasa YP, Khushoo A, Srivastava L, Mukherjee KJ. Kinetic studies of constitutive human granulocyte-macrophage colony stimulating factor (hGM-CSF) expression in continuous culture of Pichia pastoris. Biotechnol Lett. 2007 Dec;29(12):1903-8. Epub 2007 Jul 25. PubMed PMID: 17653623.
71Huo X, Liu Y, Wang X, Ouyang P, Niu Z, Shi Y, Qiu B. Co-expression of human protein disulfide isomerase (hPDI) enhances secretion of bovine follicle-stimulating hormone (bFSH) in Pichia pastoris. Protein Expr Purif. 2007 Aug;54(2):234-9. Epub 2007 Mar 31. PubMed PMID: 17498970.
72Tolner B, Smith L, Begent RH, Chester KA. Production of recombinant protein in Pichia pastoris by fermentation. Nat Protoc. 2006;1(2):1006-21. PubMed PMID: 17406338
73Hamilton SR, Davidson RC, Sethuraman N, Nett JH, Jiang Y, Rios S, Bobrowicz P, Stadheim TA, Li H, Choi BK, Hopkins D, Wischnewski H, Roser J, Mitchell T, Strawbridge RR, Hoopes J, Wildt S, Gerngross TU. Humanization of yeast to produce complex terminally sialylated glycoproteins. Science. 2006 Sep 8;313(5792):1441-3. PubMed PMID: 16960007.
74Hao Y, Chu J, Wang Y, Zhang S, Zhuang Y. Expression and aggregation of recombinant human consensus interferon-alpha mutant by Pichia pastoris. Biotechnol Lett. 2006 Jun;28(12):905-9. Epub 2006 May 31. PubMed PMID: 16786276.
75Feng MQ, Cai QS, Song DX, Dong JB, Zhou P. High yield and secretion of recombinant human apolipoprotein AI in Pichia pastoris. Protein Expr Purif. 2006 Apr;46(2):337-42. Epub 2005 Dec 12. PubMed PMID: 16516487.
76Hu S, Li L, Qiao J, Guo Y, Cheng L, Liu J. Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris. Protein Expr Purif. 2006 May;47(1):249-57. Epub 2005 Dec 13. PubMed PMID: 16403645.
77Qu J, Li X, Huang B. Production of recombinant human microplasminogen and pilot study in inducing posterior vitreous detachment. Curr Eye Res. 2005 Oct;30(10):881-9. PubMed PMID: 16251125.
78Kobayashi K, Kuwae S, Ohya T, Ohda T, Ohyama M, Ohi H, Tomomitsu K, Ohmura T. High-level expression of recombinant human serum albumin from the activation. J Biosci Bioeng. 2000;89(1):55-61. PubMed PMID: 16232698.
79Vervecken W, Kaigorodov V, Callewaert N, Geysens S, De Vusser K, Contreras R. In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris. Appl Environ Microbiol. 2004 May;70(5):2639-46. PubMed PMID: 15128513; PubMed Central PMCID: PMC404441.
80Lenassi Zupan A, Trobec S, Gaberc-Porekar V, Menart V. High expression of green fluorescent protein in Pichia pastoris leads to formation of fluorescent particles. J Biotechnol. 2004 Apr 8;109(1-2):115-22. PubMed PMID: 15063619.