Pichia Classic Protein Expression System
The Pichia Classic System is the most cost-effective eukaryotic protein expression system for both secretion and intracellular expression. It is ideally suitable for large-scale production of recombinant eukaryotic proteins. Pichia Classic utilizes stable and durable production strains to produce recombinant proteins. Pichia can be used for high-yield, high-productivity protein processing, thus dramatically reducing the associated costs and inherent time requirements of mammalian expression systems.
Pichia Classic Advantages
- Capable of making complex proteins: Has the eukaryotic subcellular machinery needed for post-translation modification including proteolytic processing, folding, disulfide bond formation and glycosylation.
- High Success Rate: Delivers a high probability of an active functional recombinant protein.
- Fast: Grows at a prolific rate and expresses protein at a high-yield per volume.
- Simple: Uses defined media formulations and established handling techniques.
- Ease of purification: Secretes proteins into the media to reduce time and money associated with protein purification and processing.
- Scalable: Can be used for small scale to commercial scale fermentation production.
- Ease in making production strains: Is easily engineered to create production strains for cell banking.
- Proven system and accepted by regulatory agencies with several therapeutic drugs approved: Produces proteins that are not contaminated with endotoxin or viruses.
Why start with E. coli? Try Pichia first!
Pichia pastoris provides an excellent alternative to E. Coli. It is a simple, fast, and cost-effective platform that results in high protein expression yield and achieves a high success rate for a variety of recombinant proteins. As a yeast, Pichia pastoris has many of the advantages of higher eukaryotic expression systems such as protein processing, protein folding, and posttranslational modification, while being as easy to manipulate as E. coli. It is faster, easier, and less expensive to use than other eukaryotic expression systems and generally gives higher expression levels. As a yeast, it has the advantages of straightforward molecular and genetic manipulations and has the added advantage of secreting the highly expressed protein into the medium.
Try Pichia Classic
The Pichia Classic Expression System can be initially tested through the purchase of various kits from RCT’s partner, Life Technologies.
Pichia services are also available from Life Technologies and a number of other service providers Try Pichia Classic
For Glycosylated Proteins, Try Pichia GlycoSwitch® Try Picha GlycoSwitch®